The gut microbiota instructs the hepatic endothelial cell transcriptome
نویسندگان
چکیده
Summary The gut microbiota affects remote organ functions but its impact on organotypic endothelial cell (EC) transcriptomes remains unexplored. liver endothelium encounters microbiota-derived signals and metabolites via the portal circulation. To pinpoint how commensals affect hepatic sinusoidal endothelium, a magnetic sorting protocol, combined with fluorescence-activated sorting, was used to isolate ECs from germ-free (GF) conventionally raised (CONV-R) mice for transcriptome analysis by RNA sequencing. This resulted in comprehensive map of microbiota-regulated EC-specific profiles. Gene Ontology revealed that several functional processes were affected. absence influenced expression genes involved cholesterol flux angiogenesis. Specifically, functioning sphingosine metabolism sphingosine-1-phosphate pathway showed drastically increased GF state. Our analyses reveal prominent role shaping transcriptional landscape endothelium.
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ژورنال
عنوان ژورنال: iScience
سال: 2021
ISSN: ['2589-0042']
DOI: https://doi.org/10.1016/j.isci.2021.103092